oxygraph plus system Search Results


oxygraph plus instrument  (Hansatech Instruments)
90
Hansatech Instruments oxygraph plus instrument
Oxygraph Plus Instrument, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dw1/ad clark-type polarographic oxygen sensor hansatech instruments oxygraph plus system  (Hansatech Instruments)
90
Hansatech Instruments dw1/ad clark-type polarographic oxygen sensor hansatech instruments oxygraph plus system
Dw1/Ad Clark Type Polarographic Oxygen Sensor Hansatech Instruments Oxygraph Plus System, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxygraph plus dw1  (Hansatech Instruments)
90
Hansatech Instruments oxygraph plus dw1
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Oxygraph Plus Dw1, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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liquid-phase oxygraph plus system  (Hansatech Instruments)
90
Hansatech Instruments liquid-phase oxygraph plus system
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Liquid Phase Oxygraph Plus System, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxygraph plus recorder  (Hansatech Instruments)
90
Hansatech Instruments oxygraph plus recorder
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Oxygraph Plus Recorder, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clarke type oxygen electrode and an oxygraph plus device  (Hansatech Instruments)
90
Hansatech Instruments clarke type oxygen electrode and an oxygraph plus device
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Clarke Type Oxygen Electrode And An Oxygraph Plus Device, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc program oxygraph plus  (Hansatech Instruments)
90
Hansatech Instruments pc program oxygraph plus
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Pc Program Oxygraph Plus, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxygraph plus data acquisition system configuration software  (Hansatech Instruments)
90
Hansatech Instruments oxygraph plus data acquisition system configuration software
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Oxygraph Plus Data Acquisition System Configuration Software, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxygraph plus control box  (Hansatech Instruments)
90
Hansatech Instruments oxygraph plus control box
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Oxygraph Plus Control Box, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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personal computer with oxygraph plus data acquisition software  (Hansatech Instruments)
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Hansatech Instruments personal computer with oxygraph plus data acquisition software
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Personal Computer With Oxygraph Plus Data Acquisition Software, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software oxygraph plus version 1.02  (Hansatech Instruments)
90
Hansatech Instruments software oxygraph plus version 1.02
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Software Oxygraph Plus Version 1.02, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pam and oxygraph plus system equipment  (Hansatech Instruments)
90
Hansatech Instruments pam and oxygraph plus system equipment
Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a <t>Oxygraph</t> Plus <t>DW1</t> (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.
Pam And Oxygraph Plus System Equipment, supplied by Hansatech Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a Oxygraph Plus DW1 (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.

Journal: Redox Biology

Article Title: Cytochrome b 5 reductase is the component from neuronal synaptic plasma membrane vesicles that generates superoxide anion upon stimulation by cytochrome c

doi: 10.1016/j.redox.2017.11.021

Figure Lengend Snippet: Correlation between NADH oxidation, O 2 consumption and superoxide anion production by soluble C b 5 R. Panel A : Representative traces of the NADH oxidation by soluble C b 5 R (2.5 and 5 μg/mL) are shown. NADH oxidase activity was measured from absorbance decay at 340 nm at 37 °C, in the following assay medium (pH 7.0): potassium phosphate 20 mM, DTPA 0.1 mM, NADH 100 μM in presence of soluble C b 5 R 2.5 (black line) and 5 μg/mL (grey line). Dotted lines indicate the slopes used to calculate the activity. Panel B : Oxygen consumption kinetics for soluble C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line). The reaction was started by addition of C b 5 R at the time marked by a large drop of trace signals. O 2 consumption was measured in presence of soluble C b 5 R using a Oxygraph Plus DW1 (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in the Panel A. Dotted lines indicate the slopes used to calculate the activity. Panel C: O 2 ·- production by soluble C b 5 R was measured with NBT. Representative traces for the kinetics of NBT reduction by soluble C b 5 R isoform, and sensitivity to SOD is shown. NBT reduction was measured at 37 °C at 560 nm with soluble C b 5 R 2.5 μg/mL in absence (black line) or presence of SOD 1 U/mL (dotted line) in the assay medium indicated in the Panel A, supplemented with NBT 200 μM. The dotted line indicates the slope used to calculate the activity. Panels D, E and F : Dependence upon C b 5 R concentration for soluble (filled squares) and membrane (open squares) C b 5 R, respectively, of NADH oxidation, oxygen consumption and superoxide production. All the results shown in this Figure are the average (± standard errors) of triplicate experiments.

Article Snippet: Panel B : O 2 consumption kinetics at 37 °C for membrane C b 5 R 1 μg/mL (black line) 5 μg/mL (grey line) measured with an Oxygraph Plus DW1 (Hansatech Instruments) electrode, filled with 2 mL of the assay medium indicated in Panel A.

Techniques: Activity Assay, Concentration Assay